Mapping of Mungbean Yellow Mosaic India Virus (MYMIV) and powdery mildew resistant gene in black gram [Vigna mungo(L.) Hepper]

Black gram, one of the important species of Asian Vigna group of grain legumes, is widely grown in South Asia and is an important source of dietary protein. The two main biological constraints particularly Mungbean Yellow Mosaic India Virus(MYMIV) and powdery mildew pose a major threat to black gram production in India. Several reports on mapping mungbean yellow mosaic virus disease and powdery mildew resistant genes on black gram using parental lines suitable for countries viz. Australia and Japan are available. However, to achieve precision in plant breeding, it is important that mapping of traits are done using parental lines which are best suited for the target area/country. Microsatellite markers facilitate effective screening of mapping population and marker assisted selection for target traits such as disease resistance in many crops. Linkage mapping for identification of genes conferring resistance to these target traits in the crop is underway. The parents selected for MYMIV mapping population are DPU 88-31 as resistant source and AKU 9904 as susceptible one. For establishment of powdery mildew mapping population RBU 38 was used as resistant and DPU 88-31 as the susceptible one. Parental polymorphism was assessed using 363 SSR and 24 RGH markers. Efforts are being made to identify the markers tightly linked to the genes responsible for resistance which will be useful for marker assisted breeding for developing MYMIV and powdery mildew resistant cultivars in black gram.